Fig. 4.

Dependence of JM–membrane interactions using engineered Put3–Neu TM–JM constructs. Sequences (A) and helical wheel diagrams (B–E) are shown of the four Put3–Neu TM–JM constructs. Deletion of residues at the junction between the Put3 sequence and the Neu TM–JM sequence leads to four different orientations of the helices in the engineered dimers. The a and d positions correspond to the helix interface. (F) Tryptophan fluorescence of the four Put3–Neu TM–JM constructs in POPC:POPS vesicles. (G) Tryptophan fluorescence of the four Put3–Neu TM–JM constructs in POPC:POPS vesicles with a molar ratio of PIP₂ to peptide of 1:1.