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. 2012 Dec 27;110(5):E415–E424. doi: 10.1073/pnas.1211850110

Fig. 6.

Fig. 6.

Elimination of Tregs and macrophages restores adjuvant vaccine efficacy. (A) The growth of recurrent TC1 tumors was decreased significantly in mice that were randomized to Ad.E7 vaccination with CD4 depletion (Combo) as compared with mice receiving either Ad.E7 or CD4-depleting antibodies alone. (B) By flow cytometry, the combination of Ad.E7 vaccination with CD4 depletion generated an increase of CD8 T cells specific to the E7 antigen in the spleen and tumor. (C) Combining vaccination with CD4 T cell depletion increased the percentage of CD8 T cells secreting IFN-γ after 6 h of in vitro stimulation with PMA/ionomycin (Bottom). (D) Immunohistochemistry of representative recurrent tumors show that Ad.E7 combined with CD4-depleting antibodies also increased the number of intratumoral CD8 T cells in recurrent lesions. *P < 0.05; **P < 0.01. (E) Mice bearing recurrent TC1 tumors that were randomized to Ad.E7 vaccination with macrophage depletion (liposomal clodronate) had significantly decreased recurrent tumor growth compared with mice receiving monotherapy.