Fig. 4.

Critical role of Aire-PHD2 in imparting immunological tolerance. (A) Range of infiltrates. Histological analysis of WT, KO, iA-WT, and iA-ΔPHD2 mice via H+E staining of fixed tissues. Mice were taken at 14 wk of age or at 15–20% of body-weight loss. i, insulitis typical of Aire-positive NOD mice. (B) Each organ (n = 10) was scored as described in Materials and Methods. (C) Representative infiltrates for female mice. White arrows indicate areas of infiltration. Magnification: 20×, except for the stomach (5×). (D) AutoAbs in female mouse serum. Each lane of the multiscreen immunoblot shows autoAbs for an individual mouse. W, Aire-WT; i, iA-WT; Δ, iA-ΔPHD2; K, Aire-KO. Dilutions, 1:25,000.