Fig. 2.

hdCK3mut and [¹⁸F]-L-FMAU PET can track reporter-labeled mouse hematopoietic cells during early engraftment and expansion in bone marrow chimera mice. (A) Lethally irradiated C57BL/6 (CD45.2) mice were transplanted with retrovirally transduced 5-FU–enriched HSCs (CD45.1). Animals were monitored for hematopoietic reconstitution over their total lifespan. MicroPET scans shown from Left,coronal; Right Upper, sagittal; Center, coronal; and Lower, transverse. (B) [¹⁸F]-L-FMAU at 4 wk posttransplant. Reporter signal observed in hdCK3mut animals in spleen (S), thymus (T), and bone marrow (BM). Probe metabolism in both cohorts seen in gastrointestinal (GI), bladder (Bl), and kidney (K). (C) [¹⁸F]-FDG MicroPET scan at 4 wk posttransplant. Nonreporter-specific signal observed in both cohorts in heart (H), spleen (S), gastrointestinal (GI), brain (Br), with metabolism in kidneys (K) and bladder (Bl). (D) In vivo accumulation of [¹⁸F]-L-FMAU in sorted hematopoietic cells from hdCK3mut animals. Reporter positive: CD45.1⁺, YFP⁺ and reporter negative: CD45.1⁺, YFP⁻. (P < 0.05).