Fig. 4.
Temperature/energy-dependent uptake of CPMV formulations. Confocal microscopy of HeLa cells and CPMV formulations (red). The cell membrane is labeled with wheat germ agglutinin (green), and the nucleus is labeled with DAPI (blue). Scale bar = 10 µm. Cells were incubated for 3 h with 106 VNPs per cell at 4 °C (left) or 37 °C (right). (A) CPMV, (B) CPMV–R5L, (C) CPMV–R5H. Imaging was performed using a Biorad 2100 confocal microscope with a 60× oil objective. Images were analyzed using ImageJ.