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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Oct 8;69(11):1000–1009. doi: 10.1002/cm.21078

Figure 1.

Figure 1

Lipid rafts are immobile throughout the cell cycle and sensitive to MLCK inhibition. (A) Lipid rafts labeled with 488-CTB showed an average fluorescence intensity increase (AFI) in the ROI of 16.29±2.42% (n=13) during interphase and 14.76±2.13% (n=17) during mitosis over 5 minutes. The diffusible lipid dye C12-Fluorescein showed rapid recovery to the ROI during both interphase and mitosis (84.79±5.98%, n=9 and 78.3±4.65%, n=8 respectively; ***p<0.0005). Error bars represent SEM. (B) Average fluorescence intensity (AFI) increase in the ROI of 488-CTB labeled cells in interphase upon treatment with cytoskeletal inhibitors. The AFI increase in the ROI of control cells (n=13) was compared to embryos treated with CytoD (n=8), Nz (n=8), ML-7 (n=9), and H1152 (n=8) (*p<0.05). (C) AFI increase in the ROI of 488-CTB labeled cells in mitosis. The AFI increase of control cells (n=17) was compared to embryos treated with CytoD (n=10), Nz (n=9), ML-7 (n=9), and H1152 (n=9) (**p<0.001). Error bars represent SEM.