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Growth of strains from the CEN.PK family on acetamide as sole nitrogen source. Expression of amdS on the multicopy plasmid pUDE158 conferred to Saccharomyces cerevisiae the ability to grow on acetamide as sole nitrogen source. The strains CEN.PK113-5D (ura3-52), IME141 [ura3-52 pAG426GPD (2μ URA3 TDH3pr-CYC1ter)], and IME142 [ura3-52 pUDE158 (2μ URA3 TDH3pr-amdS-CYC1ter)] were grown on YPD, SM and SM-Ac media and incubated at 30 °C. The plates were read after 3 days.
