Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sequential gene deletions methodology. (A) Cassette design for targeted gene deletion and seamless marker removal. (B) Experimental procedure for the sequential deletion of CAN1 and ADE2 in Saccharomyces cerevisiae by amdSYM recycling. (C) Sequencing results of the CAN1 loci of the marker-free IMX206 (can1Δ ade2Δ).
