Figure 2.

a) Chemical structure of the self-quenched fluorescence dye, calcein. b) Kinetics of cargo release from DBC-tagged vesicles induced by ¹O₂ formation: r22-b-PPO/DPhyPC liposomes hybridized with c22-BMI at different lipid/DBC ratios (curves 1–4: 1048, 2316, 5321, and 9271, respectively); r22-b-PPO/DPhyPC liposomes in the absence of c22-BMI (curve 5); and c22-b-PPO/DPhyPC liposomes in the presence of c22-BMI (curve 6). c) Light-induced sequence-specific release of calcein from a mixture of c22-BMI/r22-b-PPO/DPhyPC liposomes and calcein-loaded DPhyPC liposomes (curve 1), and a mixture of calcein loaded c22-BMI/r22-b-PPO/DPhyPC liposomes and non-loaded DPhyPC liposomes (curve 2). All samples were irradiated for 104 min.