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. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: J Immunol. 2010 Oct 15;185(10):5859–5868. doi: 10.4049/jimmunol.1001187

Figure 2. Primary IgM and IgG AFC responses in the presence or absence of Mer.

Figure 2

The number of splenic NP-specific IgM (A, upper panel) and IgG (B, lower panel) secreting AFCs were measured by ELISpot assay 9, 14 and 21 days after immunization of B6 and Mer−/−mice with NP-CGG. Each circle represents the number of AFCs per 1×106 splenocytes obtained from an individual mouse. Open and closed circles represent data from B6 and Mer−/− mice, respectively. Horizontal bars represent the average number of AFCs. Statistical analysis was performed by Student's t-test. (C) A representative image of an ELISpot assay obtained on day 14 of the anti-NP response in six B6 (B1–6, left) and six Mer−/− (M1–6, right) mice is shown. As described in Materials and Methods, one million (1×106) splenocytes were plated in the first row and subsequently two-fold serial dilution was carried out for each sample (indicated in the left side of the image as 1, 1/2 and 1/4). These data were obtained from age and sex-matched five to six mice of each genotype.