Skip to main content
. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: J Immunol. 2010 Oct 15;185(10):5859–5868. doi: 10.4049/jimmunol.1001187

Figure 3. Enhanced GC responses of B cells deficient in Mer.

Figure 3

(A) Splenocytes obtained on days 9 and 14 post-immunization from NP-CGG immunized B6 (open circle) and Mer−/− (closed circle) mice were stained with B220 and the GC B cell marker PNA. The percentage of B220+PNA+ GC B cells is shown. Horizontal bars represent the average percentage of GC B cells. (B) Spleen sections obtained from B6 and Mer−/− mice on day 14 of the NP-CGG response were stained with the GC marker PNA. The number of small, medium and large GCs per 100× magnification field was counted from randomly chosen areas on spleens from B6 (open bar) and Mer−/− (shaded bar) mice. GCs in two randomly chosen fields per mouse spleen were counted. Statistical analysis was performed by Student's t-test. These data were obtained from three to five mice of each genotype. (C) Splenocytes obtained on days 9 (left), 14 (middle) and 21 (right) post-immunization from NP-CGG immunized B6 (open) and Mer−/−(closed) mice were stained with B220 and the GC B cell marker PNA. The percentage of B220+PNA+ GC B cells for each time point is shown. These data were obtained from five to six mice of each genotype.