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. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: J Immunol. 2010 Oct 15;185(10):5859–5868. doi: 10.4049/jimmunol.1001187

Figure 6. Mer expressing cells within GCs do not co-localize with FDCs.

Figure 6

(A): Immunohistological analysis of spleen sections, obtained from B6 (top row) and Mer−/− mice (bottom row) on day 14 after NP-CGG immunization, was performed using PNA (green), anti-Mer (red) and anti-CD3 (blue). In the 1st column, PNA+ (green) GC area is shown in dotted red line. In the 2nd column, the localization of Mer expressing cells within the GC marked by the dotted green line is shown. In the 3rd column, the presence of follicular helper T cells within the GC is also shown in a similar green area. In the 4th column, two color overlay image of anti-Mer (red) and anti-CD3 (blue) staining is shown. In the 5th column, the distribution of Mer expressing cells in GCs, T cell zone and in the red pulp areas are shown in the three color overlay image. Arrow indicates the accumulation of Mer+ cells at the border between GC and T cell zone. (B) Similar immunohistology analysis as in Panel A was performed in which two adjacent spleen sections were analyzed. One section was co-stained with anti-CD21/35 (green) and anti-Mer (red) and the other was stained with anti-CD21/35 (green) and FDC marker FDC-M1 (red). (C) Similar analysis as in Panel B was performed in which anti-CD21/35 (green) staining was replaced by 2.4G2 (FcγRII/III). Original magnification of images was 200×. These data were obtained from four to five mice of each genotype.