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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Cell. 2012 Mar 30;149(1):146–158. doi: 10.1016/j.cell.2012.02.042

Figure 2. β-gal labels Lrig1+ cells in the SC niche, persists in the long-term and labeling differs in Lgr5-reporter mice.

Figure 2

(A) One day after tamoxifen induction, 50% of small intestinal crypts and 40% of colonic crypts are labeled. At 90 days, the number of labeled crypts decreases to 18% in the small intestine and 10% in the colon. (B-C) Frequency of β-gal+ cells at different positions relative to the small intestinal (B) and colonic (C) crypt base one day after tamoxifen administration. (D-I) Co-staining with various differentiation markers to confirm multipotency of progeny of β-gal+ cells: Mucin2 (Muc 2) marks goblet cells (D,G); Chromogranin-A (Chr A) marks enteroendocrine cells (E,I); Lysozyme (Lys) marks Paneth cells (F); and Carbonic Anhydrase IV (CAIV) marks enterocytes (H). (J-M) Whole-mount view of Lrig1-CreERT2/+;R26RLacZ/+ and (N-Q) Lgr5-EGFP-IRES-CreERT2;R26RLacZ/+ lineage-labeled intestines. Error bars represent s.e.m. Scale bars in D-I represent 25μm and J-Q represent 500μm. See also Figure S2.

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