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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Exp Neurol. 2012 Nov 26;240:9–16. doi: 10.1016/j.expneurol.2012.11.018

Fig. 5.

Fig. 5

Effects of NBO, minocycline and their combination on AIF induction in the ischemic brain after 90-min MCAO and 48 hrs of reperfusion. Western blot was conducted to detect AIF protein in the nonischemic (NI) and ischemic (I) hemispheric tissue. Veh: vehicle; Mino: minocycline. (A) A representative western blot revealed AIF changes in the ischemic brain of each group (upper panel). The membrane was stained with Coomassie blue as a loading control (lower panel). (B) AIF protein was quantitated after normalization to the intensity of Coomassie staining and expressed as hemispheric ratio (ischemic/nonischemic). Cerebral ischemia and reperfusion induced a 0.94-fold increase in AIF protein in the ischemic tissue, which was almost completely inhibited by the combination therapy (*P < 0.05 versus Air + Veh). NBO or minocycline alone resulted in a small but not significant reduction in AIF increase. All three treatments did not affect AIF level in the nonischemic hemisphere. Data are expressed as mean ± SEM, n = 8 for each group.