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. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: Annu Rev Anal Chem (Palo Alto Calif). 2009;2:409–433. doi: 10.1146/annurev-anchem-060908-155146

Figure 1.

Figure 1

The most commonly employed diazotization reaction (Griess assay). Under aerobic conditions nitric oxide (NO) reacts to form nitrite (NO2−), which reacts with sulfanilic acid to form a diazonium salt intermediate. The diazonium salt is then coupled to N-(1-naphthyl)ethylenediamine to form the stable water-soluble azo dye (λmax ≈ 540 nm).