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. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: Annu Rev Anal Chem (Palo Alto Calif). 2009;2:409–433. doi: 10.1146/annurev-anchem-060908-155146

Figure 2.

Figure 2

Schematic diagram of a chemiluminescence-based nitric oxide analyzer. An intert gas (e.g., nitrogen (N2)) is used to both deoxygenate sample buffer and carry NO from the sample flask though a cold trap (to remove water vapor) into a reaction cell within the nitric oxide analyzer. In the reaction cell, nitric oxide (NO) reacts with ozone (O3) to form excited-state nitrogen dioxide (NO2*), which emits a photon (i.e., chemiluminescence) upon its relaxation to the ground state (NO2). Emitted light passes through an optical filter and is detected by a photomultiplier tube (PMT).