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. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: J Virol Methods. 2010 Nov 16;171(1):299–302. doi: 10.1016/j.jviromet.2010.11.005

Fig. 1.

Fig. 1

Cell lines that stably express BirA. Western blot analysis of BirA expression in SupT1, SupT1.BirA, and the previously described 293T.BirA cell lines (top panel). Cells were lysed with M-PER solution (Pierce Biotechnology, Rockford, IL USA), the lysates clarified by centrifugation and separated by SDS-PAGE. BirA was detected by Western blot using an anti-BirA antibody (Genway Biotech, San Diego, CA USA) followed by an anti-chicken IgY-HRP conjugated secondary antibody. A control Western blot for Actin (Santa Cruz Biotechnology, Santa Cruz CA USA) was also performed as a normalization control (lower panel).