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. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: J Virol Methods. 2010 Nov 16;171(1):299–302. doi: 10.1016/j.jviromet.2010.11.005

Fig. 3.

Fig. 3

Replication and biotinylation of HIV-2 viruses with the BAS inserted into Vpx and Vpr. (A) Replication assays of wild-type (ES) and BAS containing viruses. The assays were performed as described in Fig. 2 legend. Error bars denote the standard deviation of the triplicate samples harvested at each timepoint. (B) Detection of biotinylated (SA-HRP) and viral proteins (indicated at left) from virus particles concentrated by ultracentrifugation. Supernatants were collected at the end of the infection and virus particles concentrated as described in the Fig. 2 legend. Data shown is representative of two independent experiments.