Skip to main content
. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Nature. 2013 Jan 31;493(7434):669–673. doi: 10.1038/nature11810

Figure 1. In vivo calcium imaging in genetically defined subsets of primary sensory neurons.

Figure 1

a, Schematic illustrating AAV infection. LSL, loxP-STOP-loxP cassette. b-e, mGCaMP3.0 expression in somata (b, c) and central afferent fibers (d, e) of MrgprD+ (b, d) or MrgprB4+ (c, e) neurons in adult mice. Dashed lines indicate lateral margin of spinal cord. Scale bars in (b, d) = 50 and 45 μm, respectively. f, schematic illustrating imaging preparation; components not to scale. g-n, Calcium transients in the central projections of MrgprD+ (g, i, k, m) or MrgprB4+ (h, j, l, n) neurons, evoked by direct application of KCl to the spinal cord (i, j) or (in a different animal) peripheral injection of α, β-methylene ATP (k, l). Colored rectangles in (g, h) indicate Regions-Of-Interest (ROIs) used in (i, j), respectively; yellow boxes are regions for background subtraction. Scale bar in (g, h) = 40 and 20 μm, respectively. Red arrows (i-l) indicate time of stimulus delivery. (m, n) Quantification of peak ΔF/F values before (open bars) vs. after (filled bars) stimulation. **, p<.01; ***, p<.001. All data in this and other figures are mean±SEM.