Skip to main content
. Author manuscript; available in PMC: 2013 Feb 4.
Published in final edited form as: Mol Cell. 2011 Oct 21;44(2):177–190. doi: 10.1016/j.molcel.2011.07.019

Fig. 5. SIRT3KO mice have high expression and activity of hepatic SCD1.

Fig. 5

(A) mRNA transcript levels were quantified by qPCR from wt and SIRT3KO mice, (*p<0.05, n=3/genotype, 3-month old mice, standard diet, ±SEM). (B) Plasma samples from 3-month old SIRT3KO and wt mice were analyzed for desaturation indices in triglyceride (TG) phospholipids (PL) and free fatty acids (FFA) by measuring palmitate (C16), palmitoleate (C16:1), stearate (C18) and oleate (C18:1) (*p<0.05, n=5/genotype, 3-month-old mice, standard diet, ±SEM). (C) Adenoviral SCD1 promoter activity in Huh7 cells after treatment with lipids extracted from plasma or liver tissue in wt or SIRT3KO (*p<0.05, n=3/genotype, 12-week-old mice, standard diet). (D) Hepatic extracts from SIRT3KO and wt mice were analyzed for acyl-CoA lipid species (*p<0.05, n=5/genotype, 12-week-old mice, standard diet). (E) Adenoviral SCD1 promoter activity in Huh7 cells after treatment with palmitate, palmitoleate, stearate, oleate (n=3/condition, ±SEM, *p<0.05) (F) Hepatic triglycerides from wt, SCD1KO, SIRT3KO, and SCD1KO/SIRT3KO (dKO) mice fed a standard (SD) or high-fat (HF) diet were measured (n=5/genotype, fed or fasted 24 h, ±SEM). (G) 2-month old wt, SIRT3KO, SCD1KO, and dKO mice fed a HFD were tested for glucose tolerance and measured for blood glucose levels; inset data represent area under the curve (AUC), ±SEM.