Figure 4.

Maximum likelihood tree showing relationships between major ITS families. Sequences are from abundant clusters (>5% of reads) derived from Roche 454 sequencing. Branch support is indicated at each node and was calculated using 500 ML replicates. SYL = N. sylvestris, KAW = N. kawakamii, TOM = N. tomentosiformis, OTO = N. otophora. Mutations giving rise to each ribotype are listed and shown in brackets after the species abbreviation. The last number indicates the number of reads in the cluster. Scale indicates the base substitutions per site.