Table 3.

Bacterial strains, plasmids, and primers used in this study

Strains, plasmids, or primers	Genotypes, properties, or sequencesa	Sources or references
Strains
Klebsiella pneumoniae CICC10011	Apr, parent strain	CICC
K. pneumoniae XZF-308	Apr, Cmr, recombinant strain of CICC10011, harbring pDK7-nox-2	This work
Escherichia coli DH5α	supE44 ∆ lacU169 (φ80 lacZ ∆ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1	TaKaRa
Plasmids
pNOX2	pMosBlue carrying a PCR product encoding NADH oxidase of S. pneumoniae	[38]
pUC18	Apr, 2686 bp	TaKaRa
pUC18-nox-2	Apr, pUC18 derivative carrying a 1380 bp DNA fragment containing the gene of nox-2, 4066 bp	This work
pDK7	Expressing vector, Cmr, 4800 bp	[39]
pXZF-308	Cmr, pDK7 derivative carrying a 1380 bp DNA fragment containing the gene of nox-2, 6180 bp	This work
Primers b
P1	5′-CGGGGTACCTAAGGAGGATATACATATGAGTAAAATCGT-3’ (Kpn I)	This work
P2	5′-CGGCTGCAGTTATTTTTCAGCCGTAAG-3’ (Pst I)	This work

^a Ap^r: ampicillin resistance; Cm^r: chloramphenicol resistance.

^b The underlined letters indicate the restriction sites.