Table 2.
Genotyping procedure for the studied SNPs
| Gene | SNP ID | Primer sequence (5′-3′) | Genotyping procedure |
|---|---|---|---|
|
SIM2 |
rs2269188 |
F: CTCACCACGAGCTACCTGAA |
RFLP analysis of PCR product using Bsu36I |
| R: GACCAGGAGAGGGTTTGGTC | |||
| rs2070650 |
F: CAGTGCCATGGCCTTTTTAGA |
DNA sequence analysis in ABI prism 3130 Genetic Analyzer using Big Dye sequencing kit v3.1 followed by analysis using Sequencing Analysis software v 5.2. Electropherograms obtained were further analyzed by Mutation Surveyor Demo V3.24 software to check for new mutation |
|
| rs79727992 |
R: CCAATACACACACAGCACCC |
||
| rs16994404 | |||
| rs77335240 | |||
| rs78455239 | |||
| rs79022672 | |||
| ETS2 | rs34373350 |
F: GGGGGTTTCCTTCCAGACT |
|
| rs11700777 |
R: CTGATTGGGAAAGTCACGTGGG |
||
| rs114481523 | |||
| rs73450556 | |||
| rs77688599 | |||
| rs60277131 | |||
| rs78391361 | |||
| rs1803557 | |||
| rs34472454 | |||
| rs113798497 | |||
| rs374575 |
F: GTCTGATCAAGAGGCCCAAG |
||
| rs2070529 |
R: CAGAACCACTGGGGAATGAG |
||
| rs2070530 | |||
| rs2070531 | |||
| rs434421 | |||
| rs8128227 | |||
| rs6517481 | |||
| rs79863249 | |||
| rs60538921 | |||
| rs7276961 | |||
| rs1051475 |
F: TGTGTTTCTCCGACAGCTCA |
||
| rs1051476 |
R: TTTCATCAAGACCCCTACCG |
||
| rs72094783 | |||
| rs116542090 | |||
| rs74551083 | |||
| rs11540409 | |||
| rs11254 |
F: CCATTCATTCGGAGAAAACG |
RFLP analysis of PCR product using TaqI |
|
| R: AAGGCCACGCAGCTAGTAAA | |||
| rs711 | F: GCAACGGCACAGCTAATTCT |
RFLP analysis of PCR product using MspI | |
| R: AAATACAACTGTTAAGGGATTCTGA |