Skip to main content
. 2013 Feb 4;8(2):e53776. doi: 10.1371/journal.pone.0053776

Figure 1. Generation of a genetically-defined, stepwise model of HMEC transformation.

Figure 1

48R expressing shRNA targeting p16 (shp16), p53 (shp53), or both (shp16-shp53) were further infected with control virus (Vec), or viruses encoding RAS alone (RAS), MYC alone (MYC), or MYC and RAS together (M/R) and assessed at passage 19 for AIG. (A) The bar graph represents the average colony number per plate of quadruplicates. Error bars represent the standard deviation. (B) Pictorial representation of the HMEC transformation model and growth in soft agar. Normal cells (left) are transformed (right) by sequential inactivation of p16 and p53 using shRNA, and over-expression of MYC and oncogenic RAS.