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. 2013 Feb 4;8(2):e53776. doi: 10.1371/journal.pone.0053776

Figure 2. Constitutive CCND1/CDK2 activity enhances the growth of 48R-shp16 cells despite elevated p53 and p21.

Figure 2

48R-shp16 cells were infected with viruses encoding CCND1/CDK2 (D1/K2), a shRNA targeting p53 (shp53), or control virus (Vec). (A) Western blot analysis comparing parental 48R passage 11 to derivative cells. (B) Population doublings of the parental 48R (diamonds), 48R-shp16 (squares), 48R-shp16-D1/K2 (crosses), 48R-shp16-shp53 (triangles), and 48R-shp16-shp53-D1/K2 (circles) cells. Cells were grown from passage 6 at the origin and infected with shp16 at passage 11 indicated by the arrow.