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. 2013 Feb 4;8(2):e53776. doi: 10.1371/journal.pone.0053776

Figure 3. Constitutive CCND1/CDK2 activity replaces p53 inactivation in the transformation of HMEC.

Figure 3

48R-shp16 cells were infected with control virus (Vec), or viruses encoding CCND1/CDK2 (D1/K2), or shRNA targeting p53 (shp53), followed by virus encoding MYC and RAS (M/R). (A) Western blot analysis comparing parental 48R passage 11 to derivative cells. (B) Derivative cells from A were assessed for AIG. The bar graph represents the average colony number per plate of quadruplicates. Error bars represent the standard deviation.