Table 1.
Summary of BglBricks-based 2ab assembly results
| Assembly stage | 1 | 2 | 3 | Totals | |||
|---|---|---|---|---|---|---|---|
| junction type |
CA |
AK |
KC |
CK |
KA |
CA |
|
| junctions made |
28 |
14 |
14 |
24 |
24 |
528 |
632 |
| colonies screened by growth phenotype |
84 |
42 |
42 |
156 |
156 |
1728 |
2208 |
| co-transformants |
3 |
1 |
11 |
12 |
7 |
77 |
111 |
| % co-transformation |
3.57 |
2.38 |
26.19 |
7.69 |
4.49 |
4.46 |
5.03 |
| colonies screened by mapping or PCR |
28 |
14 |
14 |
48 |
48 |
622 |
774 |
| colonies w/ correct map or PCR phenotype |
26 |
13 |
13 |
47 |
48 |
535 |
682 |
| colonies w/ questionable map or PCR phenotype |
2 |
1 |
1 |
0 |
0 |
75 |
79 |
| colonies w/ incorrect map or PCR phenotype |
0 |
0 |
0 |
1 |
0 |
12 |
13 |
| colonies sequenced |
9 |
1 |
14 |
9 |
8 |
73 |
114 |
| colonies w/ correct map or PCR sequenced |
7 |
1 |
13 |
9 |
8 |
65 |
103 |
| correct sequencing result |
7 |
1 |
13 |
9 |
8 |
65 |
103 |
| colonies w/ questionable map or PCR sequenced |
2 |
1 |
1 |
n/a |
n/a |
4 |
8 |
| correct sequencing result |
0 |
1 |
0 |
n/a |
n/a |
3 |
4 |
| colonies w/ incorrect map or PCR sequenced |
n/a |
n/a |
n/a |
0 |
n/a |
4 |
4 |
| correct sequencing result |
n/a |
n/a |
n/a |
n/a |
n/a |
0 |
0 |
| % correct assembly | 92.86 | 100 | 92.86 | 97.92 | 100 | 95.06 | 96.45* |
*Average of % assembly rates for every junction at various assembly stages.