Table 2.
Summary of BBF RFC10-based 2ab assembly results
| Assembly stage | 1 | Totals | |||||
|---|---|---|---|---|---|---|---|
| junction type |
CA |
AK |
KC |
CK |
KA |
AC |
|
| total junctions made |
1 |
1 |
1 |
1 |
1 |
1 |
6 |
| colonies screened by growth phenotype |
200 |
198 |
201 |
200 |
199 |
201 |
1199 |
| co-transformants |
29 |
26 |
27 |
37 |
24 |
31 |
174 |
| % co-transformation |
14.50 |
13.13 |
13.43 |
18.5 |
12.06 |
15.42 |
14.51 |
| total colonies counted |
580 |
1011 |
274 |
366 |
688 |
567 |
3486 |
| white colonies |
45 |
83 |
24 |
34 |
48 |
31 |
265 |
| green colonies |
535 |
928 |
250 |
332 |
640 |
536 |
3221 |
| total colonies sequenced |
5 |
6 |
5 |
2 |
7 |
1 |
26 |
| white colonies sequenced |
5 |
6 |
5 |
2 |
7 |
1 |
26 |
| correct sequencing result |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
| green colonies sequenced |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
| correct sequencing result |
n/a |
n/a |
n/a |
n/a |
n/a |
n/a |
0 |
| % correct assembly | 92.24 | 91.79 | 91.24 | 90.71 | 93.02 | 94.53 | 92.40 |