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. 2013 Feb 4;8(2):e55595. doi: 10.1371/journal.pone.0055595

Figure 3. Detection of ZnT8-specific Th1 cells by intracytoplasmic cytokine expression assay.

Figure 3

PBMCs were stimulated with the identified peptides in the presence of anti-CD28/CD49d antibody and brefeldin A. After 6 h stimulation, the expression of intracytoplasmic cytokines was analyzed by flow cytometry. Gated to CD3+CD4+ cells. (A) Expression of IFN-γ and IL-2 in CD4+ T cells (right) and IFN-γ in CD4+CD45RO+ memory T cells (left) specific for ZnT8 peptides in HS#4. (B) Expression of IFN-γ and IL-2 in CD4+ T cells (right) and IFN-γ in CD4+CD45RO+ memory T cells (left) specific for ZnT8 epitopes in HS#7.