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. 2013 Feb 4;8(2):e55595. doi: 10.1371/journal.pone.0055595

Figure 5. Islet-antigen-specific T cells identified by the Direct assay display higher avidities.

Figure 5

(A) Left: The results of the Direct assay and the Cytokine-driven assay with ZnT8 single peptides from C#11 in HS#7. Right: PBMCs were cultured with titrated concentrations of the indicated peptides (0.04–25 µM) in the presence of IL-2 (days 2–7), and re-stimulated cells at a constant concentration (25 µM). IFN-γ secretion after re-stimulation. (B) Left: The results of the Direct assay and the Cytokine-driven assay with GAD65 single peptides from C#10 in HS#2 (top) and with ZnT8 single peptides from C#11. Right: PBMCs were cultured with titrated concentrations of the indicated peptides in the presence of IL-2 (days 2–7), and re-stimulated cells with 25 µM peptide. IL-13 secretion after re-stimulation.