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. 2012 Dec 11;1:23. doi: 10.1186/2046-2530-1-23

Figure 2.

Figure 2

Primary cilia are reduced in number in the endocardial cushions of cbs mutants. (A) Immunofluorescence analysis of primary cilia (arrowheads) in atrioventricular endocardial cushions of E12.5 wild type (+/+) and cbs/cbs heart using antibodies recognizing the ciliary axoneme (anti-acetylated α-tubulin, αTub, red) and the basal body (anti-γ-tubulin, γTub, green). (B) Individual cilia in E12.5 wild type (+/+) and cbs/cbs endocardial cushions, labelled with antibodies recognizing Arl13b (red) to mark the ciliary axoneme and γ-tubulin (green) to mark the basal bodies. (C, D) Immunofluorescence analysis of proliferation (C) and apoptosis (D) in endocardial cushions of E12.5 wild type (+/+) and cbs/cbs heart using antibodies recognizing phosphorylated histone H3 (C, PΗ3, yellow) and cleaved, activated caspase-3 (D, Casp3, green). (A to D) DAPI-labeled nuclei in blue. Scale bars: A, 10 μm; B, 1 μm C, D, 20 μm. (E to H) Quantification of primary cilia and basal body density (E, G) as well as proliferation (PH3+ cells) and apoptosis (caspase-3+ cells) (F, H) in atrioventricular endocardial cushions (E, F) and pericardium (G, H), respectively, of E12.5 wild type (+/+) and cbs/cbs heart. *P <0.05, **P <0.01, ***P <0.001, Student’s t test, n >3 for all comparisons. cbs, cobblestone; DAPI, 4'-6-diamidino-2-phenylindole, E12.5; embryonic day 12.5.