Table 2. Binding characteristics and stability data of DFNB31_1 wild type and mutants.
KDapp PtdIns(4,5)P2 (µM) | KD * FTDTHL (µM) | ΔΔGD-N * (kcal/mol) | |
DFNB31_1 | 7.8±2 | 46±6 | – |
DFNB31_1/R144E | >20 | 82±3 | −0.65±0.05 |
DFNB31_1/R145E | 15±5 | 71±8 | −0.53±0.05 |
DFNB31_1/K206E | 8.2±2 | 111±8 | 0.41±0.03 |
DFNB31_1/K209E | >20 | 175±15 | −0.15±0.01 |
DFNB31_1/K212E | >20 | 220±40 | 1.2±0.1 |
A Y167W mutation was introduced in DFNB31_1 and mutants thereof to function as a fluorescent probe.