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. 2013 Feb 4;8(2):e54581. doi: 10.1371/journal.pone.0054581

Table 2. Binding characteristics and stability data of DFNB31_1 wild type and mutants.

KDapp PtdIns(4,5)P2 (µM) KD * FTDTHL (µM) ΔΔGD-N * (kcal/mol)
DFNB31_1 7.8±2 46±6
DFNB31_1/R144E >20 82±3 −0.65±0.05
DFNB31_1/R145E 15±5 71±8 −0.53±0.05
DFNB31_1/K206E 8.2±2 111±8 0.41±0.03
DFNB31_1/K209E >20 175±15 −0.15±0.01
DFNB31_1/K212E >20 220±40 1.2±0.1
*

A Y167W mutation was introduced in DFNB31_1 and mutants thereof to function as a fluorescent probe.