Table 2. Ex vivo expansion: Summary of product characteristics before and after expansion.
NO. | Before expansion | After expansion | Composition of Transplanted Grafts | |||||||||
Volume of CB (ml) | MNC×107 | % CD41+ | Expansion time (day) | MNC×107 | % CD41+ | AbsoluteCD41+(×106) | Cell Viability | Fold expansion | Body Weight | MNC per kg b.w. (×106) | CD41+ cells per kg b.w. (×106) | |
1 | 78 | 4.5 | 6 | 11 | 58.5 | 52 | 304 | 91.4% | 113 | 70 | 8.4 | 4.3 |
2 | 54 | 8 | 11 | 5 | 48 | 26 | 125 | 94.0% | 14 | 61 | 7.9 | 2.0 |
3 | 69 | 24 | 4 | 7 | 264 | 33 | 871 | 98.7% | 91 | 67 | 39.4 | 13.0 |
4 | 47 | 4.6 | 8 | 6 | 611 | 22 | 1344 | 98.0% | 365 | 57 | 107.2 | 23.6 |
5 | 93 | 8.1 | 3 | 8 | 72.9 | 27 | 197 | 98.9% | 81 | 92 | 7.9 | 2.1 |
6 | 82 | 16 | 8 | 7 | 128 | 22 | 282 | 99.0% | 22 | 54 | 23.7 | 5.2 |
7 | 106 | 18 | 6 | 4 | 54 | 13 | 70 | 99.9% | 6 | 60 | 9.0 | 1.2 |
8 | 52 | 4.6 | 9 | 17 | 87.4 | 64 | 559 | 97.1% | 135 | 81 | 10.8 | 6.9 |
9 | 77 | 1.9 | 13 | 20 | 32.3 | 55 | 178 | 92.2% | 7 | 67 | 4.8 | 2.7 |
10 | 62 | 6 | 6 | 4 | 36 | 9 | 32 | 99.0% | 9 | 57 | 6.3 | 0.6 |
11 | 70 | 5 | 12 | 12 | 130 | 72 | 936 | 87.8% | 156 | 79 | 16.5 | 11.8 |
12 | 77 | 7 | 5 | 14 | 119 | 67 | 797 | 93.0% | 228 | 67 | 17.8 | 11.9 |
13 | 72 | 10 | 6 | 4 | 20 | 8 | 16 | 95.0% | 3 | 63 | 3.2 | 0.3 |
14 | 78 | 9 | 9 | 12 | 198 | 77 | 1525 | 91.0% | 188 | 70 | 28.3 | 21.8 |
15 | 86 | 10 | 8 | 14 | 310 | 54 | 1674 | 84.4% | 209 | 86 | 36.0 | 19.5 |
16 | 99 | 15 | 6 | 7 | 120 | 23 | 276 | 93.7% | 31 | 82 | 14.6 | 3.4 |
17 | 56 | 11 | 5 | 4 | 44 | 13 | 57 | 98.0% | 10 | 78 | 5.6 | 0.7 |
18 | 93 | 9 | 5 | 8 | 135 | 37 | 500 | 84.0% | 111 | 80 | 16.9 | 6.2 |
19 | 89 | 12 | 8 | 13 | 192 | 58 | 1114 | 95.1% | 116 | 66 | 29.1 | 16.9 |
20 | 70 | 20 | 5 | 13 | 380 | 63 | 2934 | 92.0% | 293 | 80 | 47.5 | 29.9 |
21 | 72 | 6 | 9 | 22 | 96 | 32 | 307 | 94.2% | 57 | 50 | 19.2 | 6.1 |
22 | 102 | 29 | 6 | 6 | 132 | 18 | 238 | 93.9% | 14 | 88 | 15.0 | 2.7 |
23 | 65 | 6.6 | 11 | 16 | 85 | 52 | 442 | 92.0% | 61 | 78 | 10.9 | 5.7 |
24 | 75 | 5.1 | 8 | 11 | 56 | 48 | 269 | 90.2% | 66 | 61 | 9.2 | 4.4 |
Median(range) | 76(47–106) | 8.55(1.9–29) | 7(3–13) | 9.5(4–22) | 107.5(20–611) | 35(8–77) | 305.5(16–2934) | 93.95%(84–99.90%) | 73.5(3–365) | 68.5(50–92) | 14.8(3.2–107.2) | 5.45(0.3–29.9) |
CB, cord blood; MNC: mononuclear cells.