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. 2013 Feb 4;8(2):e54941. doi: 10.1371/journal.pone.0054941

Table 2. Ex vivo expansion: Summary of product characteristics before and after expansion.

NO. Before expansion After expansion Composition of Transplanted Grafts
Volume of CB (ml) MNC×107 % CD41+ Expansion time (day) MNC×107 % CD41+ AbsoluteCD41+(×106) Cell Viability Fold expansion Body Weight MNC per kg b.w. (×106) CD41+ cells per kg b.w. (×106)
1 78 4.5 6 11 58.5 52 304 91.4% 113 70 8.4 4.3
2 54 8 11 5 48 26 125 94.0% 14 61 7.9 2.0
3 69 24 4 7 264 33 871 98.7% 91 67 39.4 13.0
4 47 4.6 8 6 611 22 1344 98.0% 365 57 107.2 23.6
5 93 8.1 3 8 72.9 27 197 98.9% 81 92 7.9 2.1
6 82 16 8 7 128 22 282 99.0% 22 54 23.7 5.2
7 106 18 6 4 54 13 70 99.9% 6 60 9.0 1.2
8 52 4.6 9 17 87.4 64 559 97.1% 135 81 10.8 6.9
9 77 1.9 13 20 32.3 55 178 92.2% 7 67 4.8 2.7
10 62 6 6 4 36 9 32 99.0% 9 57 6.3 0.6
11 70 5 12 12 130 72 936 87.8% 156 79 16.5 11.8
12 77 7 5 14 119 67 797 93.0% 228 67 17.8 11.9
13 72 10 6 4 20 8 16 95.0% 3 63 3.2 0.3
14 78 9 9 12 198 77 1525 91.0% 188 70 28.3 21.8
15 86 10 8 14 310 54 1674 84.4% 209 86 36.0 19.5
16 99 15 6 7 120 23 276 93.7% 31 82 14.6 3.4
17 56 11 5 4 44 13 57 98.0% 10 78 5.6 0.7
18 93 9 5 8 135 37 500 84.0% 111 80 16.9 6.2
19 89 12 8 13 192 58 1114 95.1% 116 66 29.1 16.9
20 70 20 5 13 380 63 2934 92.0% 293 80 47.5 29.9
21 72 6 9 22 96 32 307 94.2% 57 50 19.2 6.1
22 102 29 6 6 132 18 238 93.9% 14 88 15.0 2.7
23 65 6.6 11 16 85 52 442 92.0% 61 78 10.9 5.7
24 75 5.1 8 11 56 48 269 90.2% 66 61 9.2 4.4
Median(range) 76(47–106) 8.55(1.9–29) 7(3–13) 9.5(4–22) 107.5(20–611) 35(8–77) 305.5(16–2934) 93.95%(84–99.90%) 73.5(3–365) 68.5(50–92) 14.8(3.2–107.2) 5.45(0.3–29.9)

CB, cord blood; MNC: mononuclear cells.