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. 2013 Feb 4;8(2):e52960. doi: 10.1371/journal.pone.0052960

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Top: Histograms of the GP values obtained from GP images of live Zebrafish embryos stained with PY3304 (A), PY3174 (B), PY3184 (C). Histograms obtained from ROIs for plasma membrane (red line) and intracellular membranes (black) were normalized to the total number of pixels. Bottom: GP images are in false color and run over the range indicated by the color bars to indicate a higher degree of membrane order (predominately colored red) in the plasma membrane compared to intracellular membranes (predominately colored green). Embryos were stained with PY3304 (left), PY3174 (middle), PY3184 (right) excited by multi-photon excitation at 1040 nm for PY3304, 900 nm for PY 3174, 1000 nm for PY 3184. Scale bars = 10 µm.