Table 1. Genes revealed by quantitative RT-PCR analysis to be differentially expressed in women with primary dysmenorrhea on the seventh day before menstruation.
Gene | Normalized ΔCt | 2∧-ΔCt | FC | ||
Nor | Dys | Nor | Dys | ||
IL1A | 13.4±0.6 | 10.6±2.3* | 9.4E-05 | 6.6E-04 | 7.1 |
IL1B | 5.5±0.3 | 3.2±2.4* | 2.2E-02 | 1.1E-01 | 4.9 |
CSF2 | 16.3±1.3 | 14.3±0.9* | 1.3E-05 | 4.9E-05 | 3.8 |
IL6 | 10.4±0.6 | 8.7±2.4* | 7.5E-04 | 2.4E-03 | 3.3 |
IL21 | 15.6±2.1 | 14.3±1.0 | 2.1E-05 | 4.8E-05 | 2.3 |
TNF | 7.2±0.3 | 5.8±1.3* | 6.9E-03 | 1.9E-02 | 2.7 |
TNFRSF11B | 17.4±0.5 | 16.2±1.0 | 5.8E-06 | 1.3E-05 | 2.3 |
IL5 | 10.8±0.3 | 9.8±0.5* | 5.7E-04 | 1.1E-03 | 2.0 |
BMP3 | 13.7±0.4 | 12.7±0.5* | 7.4E-05 | 1.5E-04 | 2.1 |
LEFTY2 | 11.3±0.4 | 12.5±0.3** | 4.0E-04 | 1.7E-04 | −2.3 |
BMP4 | 13.7±0.9 | 15.0±0.2* | 7.4E-05 | 3.0E-05 | −2.5 |
Normalized ΔCt = Ct (GOI) – avg. (Ct (HKG)), where GOI is each gene of interest, and HKG are the housekeeping genes. Fold change (FC) = 2−ΔΔCt = 2−ΔCt (Dys) ÷ 2−ΔCt (Nor).