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. 2013 Feb 4;8(2):e55200. doi: 10.1371/journal.pone.0055200

Table 1. Genes revealed by quantitative RT-PCR analysis to be differentially expressed in women with primary dysmenorrhea on the seventh day before menstruation.

Gene Normalized ΔCt 2∧-ΔCt FC
Nor Dys Nor Dys
IL1A 13.4±0.6 10.6±2.3* 9.4E-05 6.6E-04 7.1
IL1B 5.5±0.3 3.2±2.4* 2.2E-02 1.1E-01 4.9
CSF2 16.3±1.3 14.3±0.9* 1.3E-05 4.9E-05 3.8
IL6 10.4±0.6 8.7±2.4* 7.5E-04 2.4E-03 3.3
IL21 15.6±2.1 14.3±1.0 2.1E-05 4.8E-05 2.3
TNF 7.2±0.3 5.8±1.3* 6.9E-03 1.9E-02 2.7
TNFRSF11B 17.4±0.5 16.2±1.0 5.8E-06 1.3E-05 2.3
IL5 10.8±0.3 9.8±0.5* 5.7E-04 1.1E-03 2.0
BMP3 13.7±0.4 12.7±0.5* 7.4E-05 1.5E-04 2.1
LEFTY2 11.3±0.4 12.5±0.3** 4.0E-04 1.7E-04 −2.3
BMP4 13.7±0.9 15.0±0.2* 7.4E-05 3.0E-05 −2.5

Normalized ΔCt = Ct (GOI) – avg. (Ct (HKG)), where GOI is each gene of interest, and HKG are the housekeeping genes. Fold change (FC) = 2−ΔΔCt = 2−ΔCt (Dys) ÷ 2−ΔCt (Nor).