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. 2013 Feb 4;200(3):271–286. doi: 10.1083/jcb.201208030

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© 2013 Wloka et al.

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Figure 4. — A small C-terminal region of Myo1 is required for its immobility during cytokinesis. (A) Myo1 motifs and the positions of myo1 mutations that are synthetically with hof1Δ. (B and C) Myo1 dynamics are not affected by the deletion of its putative nonhelical region. Half (B) and full (C) rings of Myo1-(AA1903Stop)-GFP in cells of the strain YEF6617 (myo1-(AA1903Stop)-GFP CDC3-RFP) were photobleached during cytokinesis. (C and D) The putative assembly domain of Myo1 is required for its immobility during cytokinesis. Half (C) and full (D) rings of GFP-Myo1-(AA1798Stop) in cells of the strain YEF6616 (GFP-myo1-(AA1798Stop) CDC3-RFP) were photobleached during cytokinesis. Except where noted, the first bleaching always corresponds to time 0, and additional bleaching (either half bleach or total bleach) is indicated by the arrowhead. (F) Expression level of the full-length and truncation alleles of MYO1. Cell lysates of strains YEF6618 (GFP-MYO1), YEF6617, YEF6616, and YEF473 (MYO1, negative control) were probed for the expression levels of Myo1 variants by Western blotting using a GFP antibody. As a loading control, the levels of the septin Cdc11 from the same cell lysates were probed using an anti-Cdc11 antibody.