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. 2013 Feb 4;200(3):271–286. doi: 10.1083/jcb.201208030

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© 2013 Wloka et al.

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Figure 8. — The immobility of Chs2 and Hof1 depends on a small region near the C-terminal end of Myo1. (A and B) Chs2 and Hof1 become mobile at the division site during cytokinesis in myo1-(AA1798Stop) cells. Chs2-GFP in strain YEF6771 (myo1-(AA1798Stop) CHS2-GFP CDC3-RFP; A) and Hof1-GFP in strain YEF6769 (myo1-(AA1798Stop) HOF1-GFP CDC3-RFP; B) were analyzed by FRAP during cytokinesis. Arrowheads denote bleaching events. Solid and dashed lines indicate the intensity measurements for the unbleached and bleached areas, respectively.