Figure 7.

Chlorogenic acid enhanced in vivo bacterial clearance. CFUs were counted in peritoneal fluid (A), blood (B), lung (C) and spleen (D) 24 h after CLP to examine whether the administration of chlorogenic acid (20 mg/kg) enhanced the clearance of CLP-induced bacteria. After serial dilutions with PBS, peritoneal fluid, blood and tissue homogenates were cultured overnight on blood-agar base plates maintained at 37°C. Data are presented as mean ± SEM; n = 8. Statistically significant differences compared with the sham group (**P < 0.01). Statistically significant differences compared with the vehicle-treated CLP group (⁺P < 0.05 and ⁺⁺P < 0.01).