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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Mol Biochem Parasitol. 2012 Dec 20;187(1):52–59. doi: 10.1016/j.molbiopara.2012.12.003

Table 1.

Sequences, melting temperatures, and amplicon lengths of the COI SNP-specific primers selected for parasite strain discrimination and quantification. Bold text denotes bases conferring specific priming – the penultimate base is a mismatch to either strain and confers additional specificity in the event that the final base, which is specific to one strain or the other, is not complementary.

Target Sequence Tm(°C) Amplicon Length (bp)
NMRI-specific primer 5' CAA AGA CTC CCA CAA AAT AAC T 59.4 127
PR-specific primer 5' CCA AAG ACT CCC ATA AAA TAA GA 59 128
Reverse primer 5' TGG TCT TCC TCG TCG TGT AA 59.3