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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Mol Biochem Parasitol. 2012 Dec 20;187(1):52–59. doi: 10.1016/j.molbiopara.2012.12.003

Table 2.

Expected and observed proportions of known cercarial mixes to validate qPCR as a means of discriminating between and quantifying parasite strains. Observed cercarial quantities were tested against expected quantities with Chi-square analysis.

Expected Proportion Mean Observed Proportion
NMRI:PR cercariae NMRI PR NMRI PR p-value
10:2 0.83 0.17 0.91 0.09 0.84
8:4 0.67 0.33 0.55 0.45 0.80
6:6 0.50 0.50 0.35 0.65 0.76
4:8 0.33 0.67 0.36 0.64 0.96
2:10 0.17 0.83 0.11 0.89 0.89