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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Exp Cell Res. 2012 Nov 27;319(4):556–565. doi: 10.1016/j.yexcr.2012.11.012

Figure 2. ATG5 and beclin 1 are required for IL-2-induced autophagy.

Figure 2

MEFs were transfected with indicated shRNA for 48 hours, and then treated with IL-2 (6000 U/ml) for 48 hours. Autophagy was assayed by Western blot analysis of LC3 expression (A) or quantitation of average number of LC3 puncta (indicated by the red arrows) per cell by confocal microscopy (B) or the number of autophagic vacuoles (indicated by the red arrows) by TEM (C) as described in the methods section (* p < 0.05).