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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jan 9;190(4):1695–1701. doi: 10.4049/jimmunol.1202443

FIGURE 3.

FIGURE 3

C. pneumoniae decreases TRAF3 in infected cells. (A) Uninfected and C. pneumoniae or C. trachomatis infected HeLa cells were either mock treated or treated with 2 μg/ml of poly(I:C) for 1 h in the absence of a transfecting reagent. Immunoblots of cell lysates were probed with anti-S32 and S36 phospho-specific IκBα mAb. Amount of protein loaded per sample was controlled by probing with anti-actin antibodies. (B) Uninfected A549 cells or cells infected with C. pneumoniae were harvested at 16, 24, 36, 48 and 64 h p.i. and probed with anti-TRAF3 or TRAF2 antibodies. Probing with anti-actin mAb was used as a loading control.