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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jan 11;190(4):1407–1415. doi: 10.4049/jimmunol.1201195

Figure 1. Human monocytes produce IL-1β in response to self dsDNA in the presence of anti-dsDNA antibody-positive serum.

Figure 1

(A–C) Measuring IL-1β in cell culture supernatants of monocytes incubated for 18 hours in the following conditions by ELISA. (A–B) Monocytes purified from a single (A) or multiple healthy donors (B, symbols indicate individual donors) were incubated with human genomic dsDNA (5 μg/ml) in the presence or absence of healthy serum or anti-dsDNA antibody-positive serum (5% final concentration) from multiple donors (A, #D1–#D4; B, #D1–#D5). (C) ANA-positive sera without anti-dsDNA antibodies (donors, #A1–#A3) were added to monocytes from a single donor in the presence or absence of dsDNA. Representative data from 2 independent experiments (A and C). *P < 0.05.