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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jan 11;190(4):1407–1415. doi: 10.4049/jimmunol.1201195

Figure 2. Anti-dsDNA antibodies are required for IL-1β production from human monocytes treated with self dsDNA.

Figure 2

(A–D) Measuring IL-1β in cell culture supernatants of monocytes incubated for 18 hours in the following conditions by ELISA. (A) Monocytes from healthy donors were incubated with dsDNA in the presence or absence of total IgG purified from anti-dsDNA antibody-positive serum or healthy serum (n = 5). (B) Monocytes were incubated with dsDNA in the presence or absence of serum depleted or un-depleted of anti-dsDNA antibodies (2 independent experiments). (C) Monocytes were treated with anti-CD32 antibodies (FcR blocker, 2.5 μg/ml) and incubated with dsDNA and anti-dsDNA antibody-positive serum (n = 5). (D) Monocytes were incubated with anti-dsDNA antibody-positive serum or dsDNA and anti-dsDNA antibody-positive serum in the presence or absence of DNase (1 μg/ml) (n = 7). Bars and error bars indicate mean and SEM, respectively. *P < 0.05.