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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jan 11;190(4):1407–1415. doi: 10.4049/jimmunol.1201195

Figure 5. Caspase-1 and NLRP3 are involved in the production of IL-1β from human monocytes in response to self dsDNA and anti-dsDNA-positive serum.

Figure 5

(A) Monocytes from a healthy donor were incubated for 7 hours with dsDNA in the presence or absence of healthy or anti-dsDNA antibody-positive serum. Active capspase-1 was measured using flow cytometry. Numbers in histograms indicate the frequency (%) of cells positive for active caspase-1. (B) IL-1β ELISA of culture supernatants from monocytes incubated for 18 hours with dsDNA and anti-dsDNA antibody-positive serum in the presence or absence of caspase-1 inhibitor (10 μM) (n = 4). (C) Measuring the NLRP3 gene expression in human monocytes incubated for 6 hours in the presence or absence of anti-dsDNA antibody-positive serum or a combination of dsDNA and anti-dsDNA antibody-positive serum by qPCR (n = 2). (D) IL-1β ELISA of culture supernatants from monocytes nucleofected with scrambled or NLRP3-specific siRNA followed by the incubation for 18 hours with dsDNA and anti-dsDNA antibody-positive serum (n = 6). Representative data from 4 independent experiments (A). Bars and error bars indicate mean and SEM, respectively. *P < 0.05.