Skip to main content
. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jan 11;190(4):1407–1415. doi: 10.4049/jimmunol.1201195

Figure 6. The production of IL-1β from human monocytes in response to self dsDNA and anti-dsDNA antibody-positive serum requires reactive oxygen species (ROS) synthesis and K+ efflux.

Figure 6

(A) Flow cytometric analysis of reactive oxygen species (ROS) in monocytes stimulated for 4 hours with dsDNA in the presence or absence of healthy or anti-dsDNA antibody-positive serum. (B–C) IL-1β ELISA of culture supernatants from monocytes incubated for 18 hours with dsDNA and anti-dsDNA antibody-positive serum in the presence or absence of the ROS inhibitor DPI (50 μM, B) or KCl (100 mM, C). Representative data from 3 independent experiments (A). Bars and error bars indicate mean and SEM, respectively (n = 5 and 7 for B and C, respectively). *P < 0.05.