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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Immunol. 2013 Jan 11;190(4):1407–1415. doi: 10.4049/jimmunol.1201195

Figure 7. Cell culture supernatant from monocytes treated with a combination of self dsDNA and anti-dsDNA antibody-positive serum promotes IL-17 production from IL-1 receptor I (IL-1RI)-positive memory CD4+ T cells in an IL-1β-dependent manner.

Figure 7

ELISA of IL-17 production from sorted human IL-1RI+ memory CD4+ T cells that were stimulated for 5 days with anti-CD3 and -CD28 antibody-coated beads in 80% culture medium with 20% of supernatants from monocytes treated for 18 hours with self dsDNA in the presence or absence of anti-dsDNA antibody-positive serum. Some IL-1RI+ memory CD4+ T cells were incubated with culture medium alone or medium with anti-CD3 and anti-CD28 antibody-coated beads in the presence or absence of recombinant IL-1β (20 ng/ml). IL-1R antagonist (100 ng/ml) was added on days 0 and 2. Bars and error bars indicate mean and SEM, respectively (n = 5). The presence and absence of each treatment are indicated by + and −, respectively. *P < 0.05.