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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Exp Cell Res. 2012 Oct 29;319(4):546–555. doi: 10.1016/j.yexcr.2012.10.005

Figure 8. Silencing α5 integrin inhibits FN matrix organization and cell cluster formation.

Figure 8

(A) Western blotting results showing α5 integrin and actin detection for cells after transfection with specific α5 or mock siRNA. (B) Extent of matrix compaction by mock and α5-silenced cells cultured 18h on collagen matrices in DMEM/FBS. Compaction was calculated as final matrix height/starting matrix height. (C) Mock and α5 integrin silenced cells were cultured for 18h on collagen matrices in DMEM/FBS. At the end of the incubations, samples were fixed and stained for actin and PI. (D) same as “C” except the cells were stained for α5 integrin and FN. Bar = 100 μm.