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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Mol Carcinog. 2011 Nov 15;52(3):195–206. doi: 10.1002/mc.21843

Figure 2.

Figure 2

Dose-and time dependent effect of silibinin on nuclear levels of p65 and p50, and cytoplasmic levels of IκBα and pIκBα in human CRC cells. (A) Cells at 60% confluency were starved for 24 h, then treated with indicated doses of silibinin (Sb) for 2 h followed by TNFα (10 ng /mL) treatment for 10 min. (B) Cells at 60% confluency were starved for 24 h, then treated for indicated time with TNFα (10 ng /mL) without or with prior treatment of silibinin (Sb) at 100 μM dose for 2 h. In general, equal amounts of protein from nuclear or cytoplasmic extracts were resolved on 12% SDS-PAGE, transferred on to a nitrocellulose membrane, probed with appropriate primary and secondary antibodies, and visualized by ECL as detailed in ‘Materials and Methods’ section. Densitometric analyses of immunoblots were done by Scion Image program (NIH, Bethesda, MD). The data shown are representative of at least two independent experiments.