FIGURE 6.

Increased IL-4 transcription in 4get mice and production of IL-4 and IL-6 by LN MCp after T. spiralis infection of BALB/c mice. A. BM, spleen, mesenteric LN, and peritoneal lavage (PL) fluid were harvested from naïve BALB/c and 4get mice, and from 4get mice on d6 post T. spiralis (Ts) infection. The single cell suspensions were assessed for GFP expression within the MC and Ba lineage populations. Histograms of GFP expression are shown for MC (left) and Ba (right) from each tissue as indicated. Histogram colors represent: gray, naïve BALB/c mice; black, naïve 4get mice; red, T. spiralis-infected 4get mice. Naïve LN data are based on very few events. B. Mean fluorescence intensities (MFI) of GFP in the indicated MC (left) or Ba (right) lineage populations from 4get mice represent mean ± SEM, n = 13–14 mice, 3 experiments. ** p < 0.0005, calculated by the Mann-Whitney U test. C. Intracellular cytokine staining for the indicated cytokines in mLN MCp d6 post infection in a representative BALB/c mouse. The gray lines are the isotype control, and black lines are the indicated cytokine. Histograms are from one mouse whose response was close to the mean shown in D. D. MFI index of intracellular cytokine staining for IL-4 and IL-6 in mLN MCp, showing individual values and mean values ± SEM (bars), d6 post infection, 13 mice per group, 5 experiments.